Patents – 3d.FAB

3D UNIPROD

Title	Publication number	Date of filling	Date of publication	Inventors	Applicants
Production of cellular products by cells embedded in a hydrogel matrix	EP4141 095A1	25 August2021	1 March 2023	L. Chastagnier, E. Petiot, E-J. Courtial, C. Marquette	Sartorius Stedim FMT, UCBL1, INSA, CPE

Abstract
Methods and systems for performing a bioproduction process are described, the bioproduction process comprising the production of a cellular product by a cell population. The methods include the steps of culturing the cell population at least partially embedded in a hydrogel matrix forming a three-dimensional structure.

Patent file

Title	Publication number	Date of filling	Date of publication	Inventors	Applicants
Methods for production of engineered cells	EP4141096A1	25 August 2021	1 March 2023	L. Chastagnier, E. Petiot	Sartorius Stedim FMT, UCBL1, INSA, CPE

Abstract
Methods and systems for performing a bioproduction process are described, the bioproduction process comprising the production of a transformed cell population. The methods include the steps of culturing a cell population at least partially embedded in a hydrogel matrix forming a three-dimensional structure, the hydrogel matrix also comprising a transforming agent.

Patent file

Title	Publication number	Date of filling	Date of publication	Inventors	Applicants
Monitoring of biofabrication processes	EP4361944A1	31 October 2022	1 May 2024	R. Sjögren, O-W. Reif, J. Trygg, E. Petiot, C. Marquette, L. Chastagnier	Sartorius Stedim FMT, UCBL1, INSA, CPE

Abstract
Methods for monitoring a process for producing multicellular structures from a cell population in a 3D cell culture are described. The methods comprise obtaining one or more 3D images of the 3D cell culture that are acquired by imaging the cell culture at different depths and/or from different angles, processing the one or more images to btain a plurality of image-derived features, and determining the value of one or more metrics indicative of the rogress or outcome of the maturation process using a statistical model adapted to predict the values of the one or more metrics indicative of the progress or outcome of the maturation process using inputs comprising the plurality of image-derived features. Related methods, systems and products are also described.

Abstract

Methods for monitoring a process for producing multicellular structures from a cell population in a 3D cell culture are described. The methods comprise obtaining one or more 3D images of the 3D cell culture that are acquired by imaging the cell culture at different depths and/or from different angles, processing the one or more images to btain a plurality of image-derived features, and determining the value of one or more metrics indicative of the rogress or outcome of the maturation process using a statistical model adapted to predict the values of the one or more metrics indicative of the progress or outcome of the maturation process using inputs comprising the plurality of image-derived features. Related methods, systems and products are also described.

Patent file